Esterasa leucocitaria e interleucina 1 Beta en fluido crevicular gingival como marcadores de enfermedad periodontal

Abstract

PURPOSE: To value the useful measurement of the leukocyte esterase and Interleukin -1beta in gingival crevicular fluid as a biologic mark predictor in the progresión of periodontal disease. METHODS: In a case-control study between August 2005 and February 2006 defining as cases patients with confirmed diagnosis of periodontal disease and controls patients with no periodontal disease. Samples from 290 patients was collected with standarized paper points positioned in the gingival sulcus. The quantification and semi-quantification of the leukocyte esterase levels and IL-1 with an ELISA assay in gingival crevicular fluid was performed.. The semiquantification of the leukocyte esterase used Multistix -10SG strips. The quantification was measured at 400 nm wave length spectrum. An absorbance factor was calculated trough leucocytes/l. An ELISA assay was performed to get the quantification of IL-1 through a commercial ELISA Kit (Peprotech). The associated risk factors , deep probbing, bone resortion and plaque index was got from the clinic data file