Participación de los metabolitos del ácido araquidónico en la inducción de migración de líneas celulares de cáncer de mama, en respuesta al estímulo con ácido linoleico

Abstract

Various epidemiological studies suggest a link between high fat intake and risk of breast cancer. Previous work has demonstrated that linoleic acid (LA), a dietary polyunsaturated fatty acid, increases the migration of breast cancer cells in vitro. However, the signal transduction pathways that regulate these events remain uncharacterized. The aim of the present study was to demonstrate whether LA induces cell migration and assess whether induces activation of key cell-migration regulatory molecules such as focal adhesion kinase (FAK) and extracellular signal-regulated kinase (ERK) in MDA-MB-231 breast cancer cells, whether this response is dependent on the activity of enzymes that metabolize arachidonic acid (AA) (LA is the main precursor of AA) such as cyclooxygenases (COX), lipoxygenases (LOX) and/or cytochrome P450 monoxygenases (CYP450)), and also dependent on the activity of Gi/G0, and Gq/11 proteins