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Methylation of the L1 gene and integration of human papillomavirus 16 and 18 in cervical carcinoma and premalignant lesions

dc.contributor.authorTorres Rojas, Francisco Israel
dc.contributor.authorAlarcón Romero, Luz Del Carmen
dc.contributor.authorLeyva Vazquez, Marco Antonio
dc.contributor.authorOrtiz Ortiz, Julio
dc.contributor.authorMendoza Catalán, Miguel Ángel
dc.contributor.authorHernández Sotelo, Daniel
dc.contributor.authorDel Moral Hernández, Oscar
dc.contributor.authorRodríguez Ruiz, Hugo Alberto
dc.contributor.authorLeyva Illades, Dinorah
dc.contributor.authorFlores Alfaro, Eugenia
dc.contributor.authorIllades_Aguiar, Berenice
dc.creatorTorres Rojas, Francisco Israel; 368312
dc.creatorAlarcón Romero, Luz Del Carmen; 259901
dc.creatorLeyva Vazquez, Marco Antonio; 264272
dc.creatorOrtiz Ortiz, Julio; 170239
dc.creatorMendoza Catalán, Miguel Ángel; 236704
dc.creatorHernández Sotelo, Daniel; 259435
dc.creatorDel Moral Hernández, Oscar; 47380
dc.creatorRodríguez Ruiz, Hugo Alberto; 557610
dc.creatorLeyva Illades, Dinorah; 443568
dc.creatorFlores Alfaro, Eugenia; 224947
dc.creatorIllades_Aguiar, Berenice;#0000-0003-3937-335X
dc.date.accessioned2019-04-04T00:02:02Z
dc.date.available2019-04-04T00:02:02Z
dc.date.issued2017-09-13
dc.identifier.issn2278-2286
dc.identifier.urihttp://ri.uagro.mx/handle/uagro/406
dc.description.abstractHigh-risk human papillomavirus (HPV) is the primary cause of cervical carcinoma (CC). Viral integration into the host chromosomes is associated with neoplastic progression, and epigenetic changes may occur as a result. The objective of the present study was to analyze HPV L1 gene methylation and to compare the use of quantitative polymerase chain reaction (qPCR), in situ hybridization (ISH) and L1 methylation analysis as methods for detecting HPV integration. Cervical scrapes or biopsy samples positive for HPV 16 or 18, from 187 female patients with CC, squamous intraepithelial lesions (SILs) or no intraepithelial lesion (non-IL) were analyzed. Methylation of the L1 gene was determined using bisulfite modification followed by PCR, and HPV integration was subsequently analyzed.
dc.description.sponsorshipThe present study was supported by the National Council of Science and Technology (grant no. 201579). The authors would like to thank Dr Marco Antonio Jiménez López of the Cancer Institute of the State of Guerrero Dr. Arturo Beltrán Ortega for the histopathological diagnosis, and the technicians of the Cytopathology and Histochemistry Laboratory (School of Chemical and Biological Sciences, Autonomous University of Guerrero) for their assistance
dc.formatpdf
dc.language.isoeng
dc.publisherOncology Letters
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectCAB Thesaurus /DNA methylation
dc.subjectCAB Thesaurus /Human papilloma virus
dc.subjectCAB Thesaurus /Biomarkers
dc.subjectCAB Thesaurus /Cervical cáncer
dc.subjectCAB Thesaurus /Squamous intraepithelial lesión
dc.subjectCAB Thesaurus /Integration
dc.subject.classificationMEDICINA Y CIENCIAS DE LA SALUD::CIENCIAS MÉDICAS::PATOLOGÍA::CARCINOGÉNESIS
dc.titleMethylation of the L1 gene and integration of human papillomavirus 16 and 18 in cervical carcinoma and premalignant lesions
dc.typeArtículo
dc.type.conacytarticle
dc.rights.accesopenAccess
dc.audiencegeneralPublic
dc.identificator3||32||3207||320703
dc.format.digitalOriginBorn digital
dc.thesis.degreelevelDoctorado
dc.thesis.degreenameDoctorado en Ciencias Biomédicas
dc.thesis.degreegrantorUniversidad Autónoma de Guerrero
dc.thesis.degreedepartmentFacultad de Ciencias Químico Biológicas
dc.thesis.degreedisciplineMedicina y Ciencias de la Salud
dc.identifier.cvuagro05091779
dc.identifier.doihttp://dx.doi.org/10.3892/ol.2017.7596


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