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dc.contributorHernandez Sotelo, Daniel
dc.contributor.advisorHERNANDEZ SOTELO, DANIEL; 259435
dc.contributor.authorLópez Martínez, Nestor José
dc.date.accessioned2023-10-31T17:06:18Z
dc.date.available2023-10-31T17:06:18Z
dc.date.issued2021-07
dc.identifier.urihttp://ri.uagro.mx/handle/uagro/4149
dc.description.abstractThe GTPase Rac1 is a member of the Rho family, which plays a fundamental role in various cellular processes such as cell migration and invasion, cell adhesion, polymerization of actin filaments. In cancer, Rac1 participates in epithelial-mesenchymal transition, angiogenesis and promoting the invasive capacity of cells. Rac1 functions at the cytoplasmic level have been widely studied, but its participation in other cellular compartments has been poorly investigated. Several studies demonstrated that Rac1 can be translocated to the nucleus; however, little is known about the mechanism of Rac1 nuclear-cytoplasm shuttling and its function in the nucleus. Rac1 nuclear import is dependent on its interaction whit proteins such as the importin karyopherin ¿2 and can be induced in response to stimuli such as epidermal growth factor. On the other hand, it is known that estrogens induce the nuclear translocation of Rac1; however, it is unknown which of the three estrogen receptors is necessary for this process. Objective: To evaluate the participation of the estrogen receptors ERa and ERB in the translocation of Rac1 to the nucleus in HaCaT cells. Methodology: HaCaT cells were transfected with a GFP-Rac1 expression plasmid and stimulated with estrogens in the presence or not of a chemical inhibitor for ERa and ERB. The subcellular localization of Rac1 was determined by fluorescence microscopy and cellular fractionation. Results: Our results indicate that the nuclear translocation of Rac1 in response to estrogens in HaCat cells, is not dependent on ERa or ERB, and suggest that it may be depend on GPR30 receptor. Conclusions: Rac1 translocates to the nucleus in estrogen-stimulated HaCaT cells. The estrogen receptors ERa or ERB, do not participate in the translocation of Rac1 to the nucleus in response to estrogens.
dc.description.sponsorshipConsejo Nacional de Ciencia y Tecnología
dc.formatpdf
dc.language.isospa
dc.publisherUniversidad Autónoma de Guerrero (México)
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.subjectRac1
dc.subjectEstrogens
dc.subjectNuclear translocation
dc.subjectEstrogen Receptors
dc.subject.classificationBIOLOGÍA Y QUÍMICA::QUÍMICA::BIOQUÍMICA::BIOLOGÍA MOLECULAR
dc.titleAnálisis de la participación del receptor de estrógenos ERa/ErB y GPR30 en la translocación nuclear de Rac1 en la línea celular HaCaT.
dc.typeTesis de maestría
dc.contributor.committeeMemberGaribay Cerdenares, Olga Lilia
dc.contributor.committeeMemberNavarro Tito, Napoleón
dc.contributor.committeeMemberRamos Payán, Rosalio
dc.type.conacytmasterThesis
dc.rights.accesopenAccess
dc.audiencegeneralPublic
dc.audience
dc.identificator2||23||2302||230221
dc.format.digitalOriginBorn digital
dc.thesis.degreelevelMaestría
dc.thesis.degreenameMaestría en Ciencias Biomédicas
dc.thesis.degreegrantorUniversidad Autónoma de Guerrero
dc.thesis.degreedepartmentFacultad de Ciencias Químico Biológicas
dc.thesis.degreedisciplineBiología y Química
dc.identifier.cvuagro10132317


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