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dc.contributorFernandez Tilapa, Gloria
dc.contributor.advisorFERNANDEZ TILAPA, GLORIA; 90223
dc.contributor.authorTacuba Saavedra, Arvey
dc.date.accessioned2023-10-31T17:06:42Z
dc.date.available2023-10-31T17:06:42Z
dc.date.issued2021-07
dc.identifier.urihttp://ri.uagro.mx/handle/uagro/4150
dc.description.abstractBackground: Dysregulation of microRNAs is associated with multiple human diseases, including cancer. Multiple studies have demonstrated the alteration of miR-411-5p and its duality of function in human cancer. Particularly in gastric cancer, it has been reported that miR-411-5p is decreased, however the role of this microRNA in this pathology is unknown, because it has not been associated with its target genes. Objective: This work aims to evaluate the effect of miR- 411-5p overexpression on the proliferation, migration and expression of GRB2 in AGS cell line. Methods: Mimic microRNAs were used to induce miR-411-5p overexpression, miR-411-5p quantification was done by two-step RT qPCR, GRB2 expression levels were determined by one-step RT qPCR, protein levels by Western Blot, migration was determined by wound closure assays, and cell proliferation through the MTT method. Results: We show that in gastric cancer, there is no significant decrease in miR-411-5p with respect to non-tumor tissue, however, we observed that the lower levels coincide with the presence of Helicobacter pylori. GRB2 shows a nonsignificant increase in gastric cancer compared to non-tumor gastric tissue. The overexpression of miR-411-5p decreases the GRB2 messenger and indirectly stabilizes its protein levels, decreases proliferation, and increases AGS cell migration. Conclusions: miR-411-5p has a downward trend in gastric cancer tissue compared to non-tumor tissue, Helicobacter pylori could potentiate the decrease of miR-411-5p in gastric cancer. The level of expression of GRB2 in gastric cancer has a tendency to increase with respect to a non-tumor tissue. The overexpression of miR-411-5p induces a decrease in the GRB2 transcript and indirectly stabilizes its protein levels, decreases proliferation and increases the migration of AGS cells.
dc.description.sponsorshipConsejo Nacional de Ciencia y Tecnología
dc.formatpdf
dc.language.isospa
dc.publisherUniversidad Autónoma de Guerrero (México)
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.subjectmiR-411-5p
dc.subjectGRB2
dc.subjectmigration
dc.subjectproliferation
dc.subjectAGS
dc.subject.classificationMEDICINA Y CIENCIAS DE LA SALUD::CIENCIAS MÉDICAS::PATOLOGÍA::ONCOLOGÍA
dc.titleEfecto de la sobreexpresión de miR-411-5p sobre el nivel de GRB2, la proliferación y migración en células AGS.
dc.typeTesis de maestría
dc.contributor.committeeMemberPeralta Zaragoza, Oscar
dc.type.conacytmasterThesis
dc.rights.accesopenAccess
dc.audiencegeneralPublic
dc.identificator3||32||3207||320713
dc.format.digitalOriginBorn digital
dc.thesis.degreelevelMaestría
dc.thesis.degreenameMaestría en Ciencias Biomédicas
dc.thesis.degreegrantorUniversidad Autónoma de Guerrero
dc.thesis.degreedepartmentFacultad de Ciencias Químico Biológicas
dc.thesis.degreedisciplineMedicina y Ciencias de la Salud
dc.identifier.cvuagro10174649


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