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dc.contributorAlarcon Romero, Luz Del Carmen
dc.contributor.advisorALARCON ROMERO, LUZ DEL CARMEN; 259901
dc.contributor.authorPriego Hernández, Víctor Daniel
dc.date.accessioned2023-10-31T17:55:59Z
dc.date.available2023-10-31T17:55:59Z
dc.date.issued2022-12
dc.identifier.urihttp://ri.uagro.mx/handle/uagro/4152
dc.description.abstractCervical cancer (CC) is the most common cancer in women in the lower genital tract. The main risk factor for developing CC is persistent infection with HPV 16. The E6 and E7 oncoproteins of HPV 16 have been related to metabolic reprogramming in cancer through the regulation of the expression and stability of HIF-1A and consequently of the expression of its target genes such as HIF1A (HIF- 1A), SLC2A1 (GLUT1), LDHA, CA9 (CAIX), SLC16A3 (MCT4) and BSG (Basigin or CD147), which are involved in glucose metabolism. This work aimed to evaluate the expression of HIF-1A, GLUT1, LDHA, CAIX, MCT4, and Basigin in patient samples and CC cell lines. To evaluate the expression level of HIF1A, SLC2A1, LDHA, CA9, SLC16A3, and BSG genes in tissue from pa-tients with CC and normal tissue, the TCGA dataset was used. To evaluate the expression level of these genes by RT-qPCR in CC cell lines, HPV negative (C-33A) and HPV 16 positive (SiHa and Ca Ski) cell lines were used. Increased expression of HIF1A, SLC2A1, LDHA, SLC16A3, and BSG was found in Ca Ski and CA9 in SiHa compared to C-33A. Similar results were observed in CC tissues compared to normal tissue obtained by bioinformatics analysis. In conclusion, the expression of HIF-1A, GLUT1, LDHA, CAIX, MCT4, and BSG genes is increased in CC and HPV 16-positive cell lines.
dc.description.sponsorshipConsejo Nacional de Ciencia y Tecnología
dc.formatpdf
dc.language.isospa
dc.publisherUniversidad Autónoma de Guerrero (México)
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.subjectHPV 16
dc.subjectHIF-1A
dc.subjectglucose metabolism
dc.subjectcervical cancer
dc.subject.classificationBIOLOGÍA Y QUÍMICA::QUÍMICA::BIOQUÍMICA::GENÉTICA BIOQUÍMICA
dc.titleExpresión de HIF-1A y genes blanco in vitro e in silico en cáncer cervicouterino.
dc.typeTesis de maestría
dc.contributor.committeeMemberIlladez Aguiar, Berenice
dc.contributor.committeeMemberMónica Espinoza, Mónica
dc.contributor.committeeMemberPeralta Zaragoza, Oscar
dc.contributor.committeeMemberHernández Sotelo, Daniel
dc.type.conacytmasterThesis
dc.rights.accesopenAccess
dc.audiencegeneralPublic
dc.identificator2||23||2302||230204
dc.format.digitalOriginBorn digital
dc.thesis.degreelevelMaestría
dc.thesis.degreenameDoctorado en Ciencias Biomédicas
dc.thesis.degreegrantorUniversidad Autónoma de Guerrero
dc.thesis.degreedepartmentFacultad de Ciencias Químico Biológicas
dc.thesis.degreedisciplineBiología y Quimica
dc.identifier.cvuagro15168116


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