Efecto de la sobreexpresión de miR-411-5p sobre el nivel de GRB2, la proliferación y migración en células AGS.

Abstract

Background: Dysregulation of microRNAs is associated with multiple human diseases, including cancer. Multiple studies have demonstrated the alteration of miR-411-5p and its duality of function in human cancer. Particularly in gastric cancer, it has been reported that miR-411-5p is decreased, however the role of this microRNA in this pathology is unknown, because it has not been associated with its target genes. Objective: This work aims to evaluate the effect of miR- 411-5p overexpression on the proliferation, migration and expression of GRB2 in AGS cell line. Methods: Mimic microRNAs were used to induce miR-411-5p overexpression, miR-411-5p quantification was done by two-step RT qPCR, GRB2 expression levels were determined by one-step RT qPCR, protein levels by Western Blot, migration was determined by wound closure assays, and cell proliferation through the MTT method. Results: We show that in gastric cancer, there is no significant decrease in miR-411-5p with respect to non-tumor tissue, however, we observed that the lower levels coincide with the presence of Helicobacter pylori. GRB2 shows a nonsignificant increase in gastric cancer compared to non-tumor gastric tissue. The overexpression of miR-411-5p decreases the GRB2 messenger and indirectly stabilizes its protein levels, decreases proliferation, and increases AGS cell migration. Conclusions: miR-411-5p has a downward trend in gastric cancer tissue compared to non-tumor tissue, Helicobacter pylori could potentiate the decrease of miR-411-5p in gastric cancer. The level of expression of GRB2 in gastric cancer has a tendency to increase with respect to a non-tumor tissue. The overexpression of miR-411-5p induces a decrease in the GRB2 transcript and indirectly stabilizes its protein levels, decreases proliferation and increases the migration of AGS cells.