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dc.contributorVences Velazquez, Amalia
dc.contributorCortes Sarabia, Karen
dc.contributor.advisorVences Velazquez, Amalia; 265192
dc.contributor.advisorCortes Sarabia, Karen; 557803
dc.contributor.authorEstrada Moreno, Ana Karen
dc.creatorEstrada Moreno, Ana Karen; 777249
dc.date.accessioned2019-04-04T00:15:05Z
dc.date.available2019-04-04T00:15:05Z
dc.date.issued2018-11
dc.identifier.urihttp://ri.uagro.mx/handle/uagro/420
dc.description.abstractIntroduction: Bacterial vaginosis (BV) is a polymicrobial infection considered as a public health problem that affects women in reproductive age; its main etiologic agent is Gardnerella vaginalis. This bacterium is divided into eight biotypes and produces several virulence factors that favor the development of BV. In addition, an increased resistance to conventional treatment has been reported, which favors the failure to treatment and disease recurrence. Objectives: Analyze the relationship between the virulence factors production, antibiotics resistance and biotypes of G. vaginalis strains associated with normal microbiota (NM) and BV. Materials and methods: We analyzed 150 strains of G. vaginalis; biotyping, biofilm, prolidase, phospholipase C and vaginolysin production were determinated. Additionally, metronidazole and clindamycin resistance was performed by the Kirby-Bauer method and secnidazole by minimum inhibitory concentration (MIC). Results: Biotypes 1 (18%), 2 (13.3%), 5 (17.3%) and 6 (51.3%) were identified. The BVassociated strains produce more biofilm (p=0.026) and have more lithic capacity (p=0.043) that NM-associated strains. No significant difference between virulence factors production and biotypes was observed, with the exception of biotype 2 in phospholipase C production (p=0.0001). BVassociated strains produce more virulence factors in comparison with normal microbiota, especially biotypes 1 and 6, and finally a high resistance rate to metronidazole (100%), secnidazole (95.3%) and clindamycin (90.6%) was observed. Conclusion: A great diversity in the virulence factors production between NM and BV-associated strains was observed, which could contribute during the development of BV; additionally, the high resistance rate may impact in the treatment failure and disease recurrence
dc.description.sponsorshipConsejo Nacional de Ciencia y Tecnología (CONACYT)
dc.formatpdf
dc.language.isoeng
dc.publisherUniversidad Autónoma de Guerrero (México)
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4
dc.subjectTesauro Descriptores en Ciencias de la Salud /Gardnerella Vaginalis
dc.subjectTesauro Descriptores en Ciencias de la Salud /Normal Microbiota
dc.subjectTesauro Descriptores en Ciencias de la Salud /Bacterial Vaginosis
dc.subjectTesauro Descriptores en Ciencias de la Salud /Virulence Factors
dc.subjectTesauro Descriptores en Ciencias de la Salud /Biotypes
dc.subjectTesauro Descriptores en Ciencias de la Salud /Sensitivity to Antibiotics
dc.subject.classificationMEDICINA Y CIENCIAS DE LA SALUD::CIENCIAS MÉDICAS::BIOQUÍMICA
dc.titleAnálisis de biotipos, factores de virulencia y sensibilidad a antibióticos en cepas de Gardnerella vaginalis asociadas y no asociadas a Vaginosis Bacteriana
dc.typeTesis de maestría
dc.contributor.committeeMemberAlarcon Romero, Luz Del Carmen
dc.contributor.committeeMemberFlores Alfaro, Eugenia
dc.contributor.committeeMemberCastro Alarcon, Natividad
dc.contributor.committeeMemberCastro_Escarpulli, Graciela
dc.type.conacytmasterThesis
dc.rights.accesopenAccess
dc.audiencegeneralPublic
dc.identificator3||32||2302
dc.format.digitalOriginBorn digital
dc.thesis.degreelevelMaestría
dc.thesis.degreenameMaestría en Ciencias Biomédicas
dc.thesis.degreegrantorUniversidad Autónoma de Guerrero
dc.thesis.degreedepartmentFacultad de Ciencias Químico Biológicas
dc.thesis.degreedisciplineMedicina y Ciencias de la Salud
dc.identifier.cvuagro08071493
dc.viewer.xmlhttp://ri.uagro.mx:8081/viewer/index.php?code=08071493


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