Methylation of the L1 gene and integration of human papillomavirus 16 and 18 in cervical carcinoma and premalignant lesions
Torres Rojas, Francisco Israel
Alarcón Romero, Luz del Carmen
Leyva Vázquez, Marco Antonio
Ortiz Ortiz, Julio
Mendoza Catalán, Miguel Ángel
Hernández Sotelo, Daniel
Del Moral Hernández, Oscar
Rodríguez Ruiz, Hugo Alberto
Leyva Illades, Dinorah
Flores Alfaro, Eugenia
Illades Aguiar, Berenice
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High-risk human papillomavirus (HPV) is the primary cause of cervical carcinoma (CC). Viral integration into the host chromosomes is associated with neoplastic progression, and epigenetic changes may occur as a result. The objective of the present study was to analyze HPV L1 gene methylation and to compare the use of quantitative polymerase chain reaction (qPCR), in situ hybridization (ISH) and L1 methylation analysis as methods for detecting HPV integration. Cervical scrapes or biopsy samples positive for HPV 16 or 18, from 187 female patients with CC, squamous intraepithelial lesions (SILs) or no intraepithelial lesion (non-IL) were analyzed. Methylation of the L1 gene was determined using bisulfite modification followed by PCR, and HPV integration was subsequently analyzed.
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